| RESEARCH ARTICLE |
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| Year : 2013 | Volume
: 6
| Issue : 2 | Page : 90-92 |
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Depletion of CD200+ hair follicle stem cells in human prematurely gray hair follicles
Sujata Mohanty1, Anil Kumar1, Jyoti Dhawan2, Vinod K Sharma2, Somesh Gupta2
1 Department of CTVS, Stem Cell Facility, All India Institute of Medical Sciences, New Delhi, India
2 Department Dermatology and Venereology, All India Institute of Medical Sciences, New Delhi, India
Correspondence Address:
Somesh Gupta
Department of Dermatology and Venereology, All India Institute of Medical Sciences, New Delhi - 110 029
India
 Source of Support: This work was supported by the Indian Association of Dermatologists Venereologists and Leprologists-L'Oreal Pigmentation Research Grant, Conflict of Interest: None  | Check |
DOI: 10.4103/0974-2077.112669
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Introduction: Melanocyte stem cells (MelSCs) are known to be depleted in gray hair follicles. Hair follicle stem cells (HFSCs) are important for maintenance of stemness of MelSCs. Methods: We compared the proportion of CD200+ (Cluster of Differentiation 200 positive) stem cells in the outer root sheath cell suspension of gray and pigmented hair follicles of three patients with the premature graying of hair. In addition, explants culture for HFSCs was also carried out from gray and pigmented hair follicles. Cultured HFSCs were also differentiated into melanocytes. Results: The mean ± SD CD200+ HFSCs population were 9.4 ± 1.4% and 3.5 ± 0.5% for pigmented and gray hair follicles, respectively ( P = 0.002). In explants culture, the growth of HFSCs from the gray hair follicle stopped at around day 20-22, whereas the growth of the cells from the pigmented follicle continued. Conclusion: CD200+ HFSCs are depleted in prematurely gray hair in the humans. CD200+ hair follicle stem cell yield is poorer in gray hair explant culture than pigmented hair explant culture. |
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