Journal of Cutaneous and Aesthetic Surgery
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   Table of Contents     
LETTER  
Year : 2013  |  Volume : 6  |  Issue : 2  |  Page : 126
Epidermal cell suspension: Achieved by incubation at room temperature


1 Department of Dermatology and Skin Science, University of British Columbia, Vancouver, British Columbia, Canada
2 National Center for Vitiligo and Psoriasis, Riyadh, Saudi Arabia

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Date of Web Publication29-May-2013
 

How to cite this article:
AlJasser MI, Mulekar SS, Mulekar SV. Epidermal cell suspension: Achieved by incubation at room temperature. J Cutan Aesthet Surg 2013;6:126

How to cite this URL:
AlJasser MI, Mulekar SS, Mulekar SV. Epidermal cell suspension: Achieved by incubation at room temperature. J Cutan Aesthet Surg [serial online] 2013 [cited 2019 May 26];6:126. Available from: http://www.jcasonline.com/text.asp?2013/6/2/126/112680


Sir,

Non-cultured autologous melanocyte transplantation is one of the surgical treatments commonly used to treat clinically stable vitiligo patients. It involves a series of steps that has been described in previous publications. [1] One of the steps involves transferring a thin skin shave biopsy sample from the thigh to a  Petri dish More Details containing 0.2% w/v trypsin solution. This is typically followed by incubation at 37°C using an incubator for 50 min. This is thought to be important for trypsin activation and subsequent separation of the epidermis from the dermis. We conducted an experiment to check the possibility of separating the epidermis from the dermis at room temperature (RT) without the need to use an incubator.

One of the authors (Mohammed I. AlJasser) volunteered to undergo the procedure. Two thin shave biopsy samples were obtained from the right thigh under local anesthesia. Both specimens were transferred to a petri dish containing 0.2% w/v trypsin solution. One specimen was incubated at 37°C using an incubator and the other was incubated at RT in an air-conditioned room. After 50 min, we were able to separate the epidermis from the dermis in both specimens [Figure 1]. The final prepared melanocyte suspension was of similar quality [Figure 2].
Figure 1: Separation process. The dermis (top) was separated from the epidermis (bottom) in both specimens. RT: Room temperature

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Figure 2: Final prepared melanocyte suspension with a similar quality in both specimens

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This simple experiment shows that the use of an incubator may not be necessary to separate the dermis from epidermis in order to prepare epidermal cell suspension. The concept has been supported by Vielkind and Crawford who demonstrated trypsinization of epithelium under various conditions. [2] This helps to simplify the melanocyte-keratinocyte transplantation procedure; thus, making it simpler by eliminating the use of an incubator. Further studies in a larger sample size are required to validate this finding.

 
   References Top

1.Mulekar SV. Long-term follow-up study of 142 patients with vitiligo vulgaris treated by autologous, non-cultured melanocyte-keratinocyte cell transplantation. Int J Dermatol 2005;44:841-5.  Back to cited text no. 1
    
2.Vielkind U, Crawford BJ. Evaluation of different procedures for the dissociation of retinal pigmented epithelium into single viable cells. Pigment Cell Res 1988;1:419-33.  Back to cited text no. 2
    

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Correspondence Address:
Smita S Mulekar
National Center for Vitiligo and Psoriasis, Riyadh
Saudi Arabia
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/0974-2077.112680

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    Figures

  [Figure 1], [Figure 2]



 

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